Additional file 1: Figure S1. Effect of RNAi each Egf ligand gene on Jhamt and Cyp15A1 expression. Figure S2. Effect of RNAi pairwise combinations of the three Egf ligand genes Jhamt and Cyp15A1 expression. Figure S3. Expression patterns of Egfr, spi, vn, Jhamt and Cyp15A1 of adult females, during the first vitellogenic cycle. Figure S4. Compare Egfr RNAi with InR RNAi. Figure S5. RNAi Egfr at the nymph stage. Figure S6. Egf ligands and Pnt RNAi at the nymph stage. Figure S7. Specificity verification of anti-JHAMT. Table S1. Primers used for qPCR, RNAi and 5′-RACE