Additional file 3 of Inflammatory stimulation of astrocytes affects the expression of miRNA-22-3p within NSCs-EVs regulating remyelination by targeting KDM3A

Abstract

Additional file 3. Figure S3: LPS-As-CM addition significantly upregulated the expression of miRNA-22 within NSC-EVs, which could inhibit the expression of KDM3A in NSCs. A. the expressions of axon-related and remyelination-related miRNAs within As-NSC-EVs and LPS-As-NSC-EVs were detected by PCR (n = 3; data are the mean ± S.D.; *p 0.05). B. the expression of the predicted genes was detected in NSCs by PCR with or without transfection of the miRNA-22 mimics (n = 3; data are the mean ± S.D.; *p 0.05). C. Dual luciferase reporter analysis showed that the luciferase activity was not altered by the transfection of miRNA-22 mimics in the HOMER1 and TGFBR1 groups (n = 3; data are the mean ± S.D.; *p 0.05)