Cell membrane Permeability changes, mitochondrial membrane potential changes, and the effect of <i>Torreya nucifera</i> on ATP production in <i>A</i>. <i>lugdunensis</i>.

Abstract

Unlike the positive control group (Triton X-100), which showed high fluorescence, the groups treated with 25 μg/mL and 50 μg/mL of T. nucifera showed significantly low fluorescence similar to that seen in the negative control group (A). In the control group, the mitochondrial membrane potential gradient was normal (B). Groups treated with 25 μg/mL (C) and 50 μg/mL (D) of T. nucifera showed the collapse of the mitochondrial membrane potential gradient indicated by the green fluorescence in the cytoplasm. The scale bar represents 20 μm. ATP production showed that the luminescence amount was significantly reduced in the groups treated with 25 μg/mL and 50 μg/mL of T. nucifera compared to that in the control group (E).</p