Developmental regulation of YFP reporter.

Abstract

(A) Flow cytometric analysis of YFP signal from WT L. major and L. major expressing YFP from the small ribosomal subunit locus under log-phase (procyclic promastigotes) or stationary-phase (includes metacyclic promastigotes) conditions. (B) Western blot analysis of YFP expression in log phase and stationary phase parasites stably transfected with YFP transgene. L. major histone H2A levels were assessed as a protein loading control. (C) The abundance of YFP mRNA in log and stationary phase parasites was determined by q-RT-PCR. *, P (PDF)</p