Rdh54 does not alter Rad54 ATPase functions at physiological ratios.

Abstract

(A). ATPase assay illustrating the fraction of Rad54 ATP hydrolysis in the presence and absence of Rad51 with and without Rdh54K318R. The bars represent the mean and standard deviation of three independent experiments. (B). Quantification of the number of fluorescently labeled Rad54 (N = 100) and Rdh54 (N = 100) molecules in yeast nucleus. The line and error bars represent the mean and 95% confidence interval of the data. The red number above the data is the mean molecules per cell. (TIF)</p