Loss of <i>RDH54</i> results in an increase in BIR outcomes.

Abstract

(A). Graph representing the plating efficiency of WT, RDH54-KanMX/RDH54-KanMX, RDH54/rdh54Δ, rdh54Δ/rdh54Δ, rdh54K318R/rdh54K318R, and rad54Δ/rad54Δ. Crossbar and error bars represent the mean and standard deviation of independent measurements. (B). Graphical representation of the fraction of cells that are red, white, or sectored after DNA double strand break repair. Strains represented here are WT, RDH54/rdh54Δ, rdh54Δ/rdh54Δ, RDH54-KanMX/RDH54-KanMX, rdh54K318R/rdh54K318R. The column and the error bars represent the mean and standard deviation of at least three independent experiments. (C). Graph representing the percentage of non-crossover outcomes for WT, RDH54-KanMX/RDH54-KanMX, RDH54/rdh54Δ, rdh54Δ/rdh54Δ, rdh54K318R/rdh54K318R. Measurements represent the mean and standard deviation of four independent experiments. (D). Graph representing the percentage of crossover outcomes for WT, RDH54-KanMX/RDH54-KanMX, RDH54/rdh54Δ, rdh54Δ/rdh54Δ, rdh54K318R/rdh54K318R. Measurements represent the mean and standard deviation of four independent experiments. (E). Graph representing the percentage of Break induced replication (BIR) outcomes for WT, RDH54-KanMX/RDH54-KanMX, RDH54/rdh54Δ, rdh54Δ/rdh54Δ, rdh54K318R/rdh54K318R. Measurements represent the mean and standard deviation of at least four independent experiments.</p