Rad51 binding site switching of Rdh54 and Rad54 alters 3’ end accessibility and post gene conversion outcomes.

Abstract

(A). Representative gel image of experiments designed to test how efficiently Rdh54, Rad54NRdh54, and Rdh54NRad54 interfere with Rad54 mediated 3’ end accessibility to Klenow fragment. The recipient DNA in this experiment was 21 nt of homologous ssDNA and 36 bp of non-homologous DNA (B). Quantification of extended D-loops for samples with Rad54, Rdh54, Rad54NRdh54, and Rdh54NRad54. The data points and error bars represent the mean and standard deviation of at least three independent experiments. (C). Graph representing the plating efficiency (+Gal/-Gal) for WT-KanMX, rad54nrdh54, and rdh54nRad54 yeast strains. The data represents the mean and standard deviation for four independent experiments. (D). Bar graphs representing the NCO (left), CO (middle), and BIR (right) outcomes for WT-KanMX, rad54nrdh54, and rdh54nrad54. The data represent the mean and standard deviation of at least four independent experiments. The WT and rdh54Δ results are reproduced from previous figures for comparison purposes.</p