Rdh54 prevents Rad54 from creating accessible 3’ end at D-loop intermediates.

Abstract

(A). Cartoon diagram illustrating the experiment to monitor the 3’ end accessibility by using Klenow (exo-) to extend the nascent D-loop. The recipient DNA used in this experiment was 21 nt of homologous ssDNA and 36 bp of duplexed non-homologous DNA (B). Representative gel illustrating D-loop extension for Rad51 alone, Rad51+Rad54 (30 nM), Rad51+Rdh54 (30 nM), and Rad51+Rad54 (30 nM) +Rdh54 (30 nM). (C). Quantification of extended D-loop fraction. The error bars represent the standard deviation for three independent experiments.</p