Analysis of RDH54 in post gene conversion recombination.

Abstract

(A). Schematic diagram illustrating assay used to monitor recombination between homologous chromosomes during a double strand DNA break. The reporter is located on chromosome XV and only one homolog has an active I-SceI site. The inactivating mutations ade2-n is shown by a red line in the diagram. If Short tract gene conversion occurs (STGC) this mutation is lost and the ADE2 becomes active. (B). Yeast colonies plated after ± induction of I-SceI using galactose. (C). Schematic diagram illustrating the potential outcomes for DNA repair via homologous recombination. In the first step gene conversion can occur through long tract or short tract DNA repair. Following cellular division, non-crossover, crossover, and break induced replication (non-reciprocal exchange) outcomes can be determined for each event. (D). Cartoon illustration of HR outcomes that can be determined from this assay. The red lines indicate DNA extension.</p