Additional file 4 of Circular RNA circPOLR2A promotes clear cell renal cell carcinoma progression by facilitating the UBE3C-induced ubiquitination of PEBP1 and, thereby, activating the ERK signaling pathway

Abstract

Additional file 4: Supplemental Fig. 4. (a, b) Bioinformatics analysis suggested that YTHDF2 expression was associated with cRCC grade (a) and stage (b) in the cRCC cohort from TCGA database. (c) Western blotting detected the YTHDF2 level after circPOLR2A knockdown or overexpression. (d) The 4 putative m6A motifs of circPOLR2A predicted by the SRAMP prediction server. (e-h) The expression of METTL3 and YTHDF2 in cRCC cells transfected with siNC, siMETTL3 or siYTHDF2. 10e, 10f, METTL3 expression; 10 g, 10 h, YTHDF2 expression. (i, j) The schematic diagram illustrated the structure of circPOLR2A-WT (i) or circPOLR2A-MUT (j)