The basic architecture of the constructs used for <i>GmTCP19L</i>-CRISPR/Cas9-mediated genome editing.

Abstract

GmU6, Glycine max U6 promoter. sgRNA, small guide-RNA. GmTCP19L-SP1/SP2, two target sites in the exon of GmTCP19L. Cas9-F/R, the primers of the detection region for Cas9. NLS, nuclear localization sequence. The bar gene driven by a CaMV 35S promoter is used as a screening marker. Kan, kanamycin resistance gene. pVS1, pVS1 replication origin. STA, pVS1 stability function. (PDF)</p