Generation of late LiS worm in medium supplemented with human serum increases in a concentration-dependent manner.

Abstract

NTS were cultured in HM supplemented with 200 U/ml Penicillin and 200 μg/ml Streptomycin as well as 1%, 5%, 10%, 20% and 50% of HSe. The percentages of the developmental stages as well as dead parasites in culture with (A) HM alone or supplemented with (B) 1%, (C) 20% and (D) 50% HSe were calculated at indicated time points and (E) their viability was scored during bright field microscopy. (F) Representative photomicrographs were taken on day 35 post transformation. Scale bar applies to all pictures shown. Arrowheads indicate dead NTS. Arrows indicate LiS (early LiS in 5% and 10% HSe) and late liver stage (in 20% and 50% HSe). Results are pooled from three independent experiments with at least three biological replicates each. ×× p ≤ 0.01 comparing HM vs. 1% HSe; ┴┴ p ≤ 0.01 comparing HM vs. 5% HSe; ˅˅ p ≤ 0.01 comparing HM vs. 10% HSe; ≠≠ p ≤ 0.01 comparing HM vs. 20% HSe; ** comparing p ≤ 0.01 HM vs. 50% HSe. HSe, human serum; s.p., score points; p.t., post-transformation; SkS, skin stage; LuS, lung stage; LiS, liver stage. (PZFX)</p