Drug sensitivity is dependent on the developmental stage of <i>S</i>. <i>mansoni</i> larvae generated <i>in vitro</i>.

Abstract

NTS were cultured and matured in HM supplemented with 200 U/ml Penicillin and 200 μg/ml Streptomycin only or additionally supplemented with 20% HSe. 100, 10 and 1 μg/ml of (A) praziquantel, (B) oxamniquine, (C) mefloquine and (D) artemether were then added to the culture 1 day p.t. to test the SkS, 7 days p.t. to test the LuS and 6 weeks p.t. to test the LiS. Viability was scored 72h a.t. Each data point is shown as a mean ± SD of three independent experiments with at least three biological replicates each. ××× p ≤ 0.001, ×× p ≤ 0.01, × p ≤ 0.05 comparing control with 1 μg/ml drug; ┴┴┴ p ≤ 0.001, ┴┴ p ≤ 0.01, ┴ p ≤ 0.05 comparing control with 10 μg/ml drug; ***p ≤ 0.001, **p ≤ 0.01 *p ≤ 0.05 control with 100 μg/ml drug. SkS, skin stage; LuS, lung stage; LiS, liver stage; PZQ, praziquantel; OXM, oxamniquine; MFQ, mefloquine; ART, artemether; s.p., score points. (PZFX)</p