Additional file 1 of Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages

Abstract

Additional file 1: Supplementary Figure S1. Expression clusters of lncRNAs during transdifferentiation. Supplementary Figure S2. Expression clusters of protein coding genes during transdifferentiation. Supplementary Figure S3. Expression profiles of lncRNAs and protein coding genes during transdifferentiation. Supplementary Figure S4. pDECKO plasmid and sequencing oligos binding scheme. Supplementary Figure S5. FACS sorting of BLaER1-Cas9 library. Supplementary Figure S6. Statistics on quantification of pgRNA representation in the screening. Supplementary Figure S7. Quantification of pgRNA distribution before and during screening. Supplementary Figure S8. Target genes disrupted by CRISPR-Cas9. Supplementary Figure S9. CEBPa and SPI1 validation at genomic level. Supplementary Figure S10. Western blot of CEBPa. Supplementary Figure S11. Individual target validation by flow cytometry. Supplementary Figure S12. lncRNA target sites and individual validations. Supplementary Figure S13. Validation of lncRNAs knockout at genomic level. Supplementary Figure S14: Epigenetic landscape of the candidate lncRNAs. Supplementary Figure S15. FURIN and NFE2 target sites and validationsat genomic level. Supplementary Figure S16. Uncropped western blots