<i>E</i>. <i>coli</i> LF82_N mice develop severe, transmural colitis following DSS-induced inflammation.

Abstract

Mice were scored at the time of necropsy for macroscopic parameters of disease including colon length (A) and macroscopic colitis score (as described in Materials and Methods) (B). Additionally, proximal colon was blindly scored for histopathologic parameters of inflammation by a pathologist (JH) (C). Representative photomicrographs of histopathological sections imaged at 40X (scale bar = 100 μm) (D). Shared letters between different groups indicate no statistical significance with an ANOVA or Kruskal-Wallis with multiple group comparisons test. Asterisk indicate statistical significance based on a direct comparison with a Mann-Whitney two-tailed test, *p ≤ 0.05, **p ≤ 0.01. p for colon length data indicates significance based on a linear regression model for 1all groups and between the 2DSS treated groups. A and B: Control n = 17, DSS n = 21, LF82A n = 21, LF82A + DSS n = 28, LF82N n = 24, LF82N + DSS n = 32; C: Control n = 13, DSS n = 12, LF82A n = 16, LF82A + DSS n = 24, LF82N n = 19, LF82N + DSS n = 29.</p