Additional file 1 of Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Hiroshi Miyazawa (403468); Katsuhito Kino (403467); Kazuaki Ohara (758778); Kyousuke Tokorodani (12124267); Masayuki Morikawa (610544); Ryuto Anabuki (12124270); Taishu Kawada (1585072); Takanobu Kobayashi (77405); Takayuki Ohshima (150051)

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Additional file 1 of Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Authors: Hiroshi Miyazawa (403468)
Katsuhito Kino (403467)
Kazuaki Ohara (758778)
Kyousuke Tokorodani (12124267)
Masayuki Morikawa (610544)
Ryuto Anabuki (12124270)
Taishu Kawada (1585072)
Takanobu Kobayashi (77405)
Takayuki Ohshima (150051)
Publication date: 15 February 2022
Publisher
Doi

Abstract

Additional file 1: Fig. S1. Construction of the 30-merUa. Two products (30-merUa1 and 30-merUa2) were obtained (Materials and methods). However, these two products equilibrate with each other and thus could not be isolated separately. In a previous report [7], Dubey, et al. revealed that Ua comprises the α- and β-anomers. The mixture of the two products (C289H370N103O176P29) was confirmed by ESI-MS (m/z 9000.727) and then was used as 30-merUa in our experiment

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Last time updated on 12/08/2022