Additional file 1 of Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin

Abstract

Additional file 1: Fig. S1. The colorimetry of culture media for high throughput screening. Fig. S2. Production of astaxanthin determined using OD515 by the evolved strains after ARTP mutation. Fig. S3. Production of astaxanthin determined using OD515 by the strains after error-prone whole-genome shuffling. Fig. S4. HPLC analysis of carotenoid products extracted from E. coli AST-4AS cultured in 2-L bioreactor. Fig. S5. Effect of CRISPR repressing of the mutated gene on the astaxanthin production. Table S1. Mutated genes identified in E. coli AST-4AS. Table S2. Primers used in this study