Knock down of <i>dbt</i> expression in mushroom body and central complex neurons caused a daytime sleep increase which is dependent on PER protein.

Abstract

(A) Loss of expression of dbt in α, β and γ lobes of mushroom body (201Y) of flies causes a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (201Y/+) = 45, n (UAS-dicer/201Y; UAS-dbtRNAi/+) = 38. (B) Average sleep amount during the daytime and nighttime with down-regulation of dbt expression with mushroom body driver 201Y. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (201Y/+) = 45, n (UAS-dicer/201Y; UAS-dbtRNAi/+) = 38. (C) Average sleep bout duration during the daytime and nighttime with down-regulated expression of dbt with mushroom body driver 201Y. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (201Y/+) = 45, n (UAS-dicer/201Y; UAS-dbtRNAi/+) = 38. (D) Activity counts/awake time during the daytime and nighttime with down-regulated expression of dbt with mushroom body driver 201Y. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (201Y/+) = 45, n (UAS-dicer/201Y; UAS-dbtRNAi/+) = 38. (E) Loss of expression of dbt in α and β lobe of mushroom body (C309) of flies causes a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbtRNAi/+) = 43, n (C309/+) = 43, n (UAS-dicer/C309; UAS-dbtRNAi/+) = 44. (F) Average sleep amount during the daytime and nighttime with down-regulation of dbt expression with mushroom body driver C309. n (UAS-dicer/+; UAS-dbtRNAi/+) = 43, n (C309/+) = 43, n (UAS-dicer/C309; UAS-dbtRNAi/+) = 44. (G) Average sleep bout duration during the daytime and nighttime with down-regulated expression of dbt with mushroom body driver C309. n (UAS-dicer/+; UAS-dbtRNAi/+) = 43, n (C309/+) = 43, n (UAS-dicer/C309; UAS-dbtRNAi/+) = 44. (H) Activity counts/awake time during the daytime and nighttime with down-regulated expression of dbt with mushroom body driver C309. n (UAS-dicer/+; UAS-dbtRNAi/+) = 43, n (C309/+) = 43, n (UAS-dicer/C309; UAS-dbtRNAi/+) = 44. (I) Loss of expression of dbt in fan-shaped body (C205) leads to a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbtRNAi/+) = 45, n (C205/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C205) = 44. (J) Average sleep amount during the daytime and nighttime with down-regulated expression of dbt with fan-shaped body driver C205. n (UAS-dicer/+; UAS-dbtRNAi/+) = 45, n (C205/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C205) = 44. (K) Average sleep bout duration during the daytime and nighttime with down-regulated expression of dbt with fan-shaped body driver C205. n (UAS-dicer/+; UAS-dbtRNAi/+) = 45, n (C205/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C205) = 44. (L) Activity counts/awake time during the daytime and nighttime with down-regulated expression of dbt with fan-shaped body driver C205. n (UAS-dicer/+; UAS-dbtRNAi/+) = 45, n (C205/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C205) = 44. (M) Loss of expression of dbt in ellipsoid body (C819) leads to a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (C819/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C819) = 46. (N) Average sleep amount during the daytime and nighttime with down-regulated expression of dbt with ellipsoid body driver C819. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (C819/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C819) = 46. (O) Average sleep bout duration during the daytime and nighttime with down-regulated expression of dbt with ellipsoid body driver C819. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (C819/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C819) = 46. (P) Activity counts/awake time during the daytime and nighttime with down-regulated expression of dbt with ellipsoid body driver C819. n (UAS-dicer/+; UAS-dbtRNAi/+) = 46, n (C819/+) = 47, n (UAS-dicer/+; UAS-dbtRNAi/C819) = 46. (Q) Sleep profile of flies with lower expression of dbt from 201Y without expression of PER protein. n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;201Y/+) = 44, n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 44. (R) Average sleep amounts of flies with lower expression of dbt from 201Y without expression of PER protein. n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;201Y/+) = 44, n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 44. (S) Sleep profile of flies with lower expression of dbt from C309 without expression of PER protein. n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;C309/+) = 48, n (per0/Y; UAS-dicer/C309; UAS-dbtRNAi/+) = 47. (T) Average sleep amounts of flies with lower expression of dbt from C309 without expression of PER protein. n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;C309/+) = 48, n (per0/Y; UAS-dicer/C309; UAS-dbtRNAi/+) = 47. (U) Sleep profile of flies with lower expression of dbt from C205 without expression of PER protein. (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;C205/+) = 47, n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/C205) = 46. (V) Average sleep amounts of flies with lower expression of dbt from C205 without expression of PER protein. (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;C205/+) = 47, n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/C205) = 46. (W) Sleep profile of flies with lower expression of dbt from C819 without expression of PER protein. n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;C819+) = 31, n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/C819) = 41. (X) Average sleep amounts of flies with lower expression of dbt from C819 without expression of PER protein. n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/+) = 48, n (per0/Y;C819+) = 31, n (per0/Y; UAS-dicer/+; UAS-dbtRNAi/C819) = 41. ns. no significant difference, *p-GAL4/+ control flies, while orange bars (or lines) represent the treatment flies. The horizontal bar below each graph presents day (white) and night (black).</p