Brown Nat Protocols Fig 2a

Abstract

Figure 2 | Comparison of hybridization strategies. a, Hybridization efficiencies plotted as the percentage of signal scored (mean with SD) in C127 cells for probes pEx and pCx34 (red and green respectively). As a lack of hybridization signals was occasionally noted in RASER-FISH samples, owing to incomplete BrdU labelling, we assessed hybridization efficiency as follows: on an allelic basis, the presence of a single signal (either green or red) deemed that allele as scorable. At that same allele, the presence (or absence) of the neighbouring signal was recorded. Hybridization frequencies were expressed as number of alleles with both red and green signals / number of all scorable alleles ×100. The hybridization frequencies shown are calculated from 501 (4 min), 715 (4 min+dry), 755 (RASER) and 218 (30 min+dry) alleles