An example of a 2DIR contour plot.

Abstract

(A) A 2DIR contour plot showing data of a 100 μm region of a mutant mouse lens tissue slice. The plot exhibits a pair of positive (red) and negative (blue) peaks that have been normalized on the diagonal at ωpump = ωprobe = 1641 cm−1. The plot also exhibits a cross peak (grey box) with a maximum at ωpump = 1632 cm-1, ωprobe = 1701 cm−1. (B) A plot of the dashed diagonal line in (A) (grey dashed). The vertical lines (green) indicate the frequencies used in determining the diagonal ratio values (1636 and 1641 cm-1). (C) A plot of the solid horizontal line in (A) (grey). A baseline (green) is subtracted to give the cross peak intensity. When structures change from native β-sheets to amyloid-like β-sheets, the cross peak intensity increases, and its position shifts to a smaller pump frequency and larger probe frequency.</p