<i>Cryab</i>-R120G mutant mouse lenses have more amyloid-like secondary structure features than wild- type mouse lenses by 2DIR image analysis.

Abstract

(A, B) Ratio image of 1636/1641 cm-1 intensities for a wild type (A) and mutant (B) mouse lens tissue sections. (C) An overlay of two example diagonal slices for the wild type (blue, from white box in A, E) and Cryab-R120G mutant (red, from white box in B, F) mouse lens sections, normalized to 1641 cm-1. The vertical lines (green) indicate the frequencies used in determining the diagonal ratio values (1636 and 1641 cm-1). (D) An overlay of two example cross peaks for the wild type (blue, from white box in A, E) and Cryab-R120G mutant (red, from white box in B, F) mouse lens sections. (E, F) Cross peak intensity image at the Cryab-R120G mutant mouse cross peak frequency (ωpump = 1632 cm-1, ωprobe = 1701 cm-1) for the wild type (E) and mutant (F) mouse lens sections.</p