An FFPE age-related cataract human lens tissue contains more amyloid-like secondary structure features than an FFPE juvenile human lens tissue by 2DIR image analysis.

Abstract

(A, B) Ratio image of 1636/1641 cm-1 intensities for a juvenile (16 years old, A) and age-related cataract (63 years old, B) human lens tissue sections. (C) An overlay of two example diagonal slices for the juvenile (yellow, from green dot in A, E) and cataract (purple, from black dot in B, F) human lens sections, normalized to 1641 cm-1. Additionally, the wild type (blue) and Cryab-mutant (red) mouse lens sections from Fig 3 are also shown for comparison. The vertical lines (green) indicate the frequencies used in determining the diagonal ratio values (1636 and 1641 cm-1). (D) An overlay of two example cross peaks for the juvenile (yellow, from green dot in A, E) and cataract (purple, from black dot in B, F) human lens sections. (E, F) Cross peak intensity image at the human cataract cross peak frequency (ωpump = 1632 cm-1, ωprobe = 1695 cm-1) for the juvenile (E) and cataract (F) human lens sections.</p