Three-dimensional epidermal culture using normal human epidermal keratinocytes (NHEKs) and normal human epidermal melanocytes (NHEMs) and NHEKs and differentiated ADSCs.

Abstract

HMB45- and tyrosinase (TYR)-positive cells were observed in the basal layer of both epidermis-like structures (A). Melanin deposits were induced by UVB irradiation in 3D epidermal culture using NHEKs and NHEMs, as well as in NHEKs and differentiated ADSCs and were detected by Fontana-Masson staining (B).</p