Photochemistry of Benzophenone Immobilized in a Major Groove of DNA:  Formation of Thermally Reversible Interstrand Cross-link

Abstract

We here report a highly site and sequence selective formation of an interstrand cross-link of BPU-containing oligomer duplexes. The cross-link was found spontaneously reverted to original oligomers upon heating, providing a new method for the temporary connection of two DNA strands