Afatinib reduces cell proliferation after MET knockdown.

Abstract

Hs746T cells were transfected with MET siRNA (MET KD) or control siRNA (Ctr). Non-transfected cells (NT) were analyzed in parallel. Cells were harvested and re-plated one day after transfection. The following day, cells were treated with 0.5 μM afatinib or 0.05% DMSO for 72 hours. The mean of three biological experiments with standard deviation is shown. Data are shown as percent of NT untreated cells (a) or as percent of untreated cells of each group (NT, Ctr, MET KD) (b). T-test was used to compare the untreated cells between the groups and to compare afatinib-treated, DMSO-treated and untreated cells within each group (NT, Ctr, MET KD). Statistically significant differences are indicated by **p<0.01.</p