P200 family protein IFI204 is highly expressed in MHV-infected BMDCs.

Abstract

(A) Schematic of the experiment. Bone marrow cells were isolated from mouse tibia and femur and cultured for 7 to 9 days in medium containing mouse GM-CSF. BMDCs were infected by MHV (MOI = 1) and collected at 18 hpi. The cells were collected and examined for transcriptome and proteome. The graph (right panel) shows the average value of three independent experiments of transcriptome. (B) Proteome, transcriptome and qRT-PCR analyses identified higher expression of Ifi204 in MHV-infected BMDCs at 18 hpi. (C and D) qRT-PCR analysis of Ifi204 and MHV mRNA7 in MHV-infected BMDCs (C) and Ifnar-/- BMDCs (D) at different time points as indicated. **P < 0.01 and ***P < 0.001. Data are representative of three independent experiments (mean ± SD in B).</p