IFI204 inhibits the production of type I IFNs in RNA virus-infected cells.

Abstract

(A) qRT-PCR analysis of Ifnb, Ifna4 and Ifna6 in NIH3T3 cells with stable knockdown of IFI204 by lentivirus-mediated shIFI204. The cells were unstimulated (US) or stimulated with SeV, poly(I:C) (10 μg/ml) or ssPolyU (10 μg/ml) as indicated for 8 hrs. (B) qRT-PCR analysis of Ifnb, Ifna4 and Ifna6 in BMDCs with knockdown of IFI204 by lentivirus-mediated shIFI204. The cells were unstimulated (US) or stimulated with MHV (MOI = 1) or SeV as indicated for 8 hrs. (C and D) ELISA analysis of the production of IFNα in IFI204-knockdown NIH3T3 cells (C) and BMDCs (D). (E) qRT-PCR analysis of Ifnb and Ifna4 in IFI16-/- and IFI16+/+ A549 cells after stimulation with SeV, poly(I:C) (10 μg/ml) or ssPolyU (10 μg/ml) for 8 hrs. US, unstimulated control. **P < 0.01 and ***P < 0.001. Data are representative of three independent experiments (mean ± SD in A-E).</p