IFI204 inhibits IRF7 binding with its promoter.

Abstract

(A) CHIP analysis of the specific binding of IRF7 and its promoter DNA. IFI204 or IFI204 mutant was co-transfected with IRF7 into Irf3−/−Irf7−/−MEFs, which were unstimulated (US) or stimulated by SeV. Gel electrophoresis of the PCR products using Ifnb promoter primers and GAPDH primers (left panel). Levels of Ifnb promoter DNA were quantified by densitometry (right panel). (B and C) EMSA analysis of IRF7 binding with its promoter region. GST-IFI204, MBP-IRF7, GST and MBP were mixed with CY5-labeled PRD I or NC sequence probes as indicated. The NC sequence, GST and MBP are negative controls. Levels of shifted DNA and free DNA were quantified by densitometry (lower panel in C). **P < 0.01 and ***P < 0.001. Data are representative of two independent experiments.</p