Accumulation of dsRNA in cells infected with vhs-deficient viruses.

Abstract

A) HFF and HeLa cells were infected for 12 hours with HSV-1 (KOS37) WT, Vhs-, US11-, Vhs-US11-, IEUS11 and IEUS11 Vhs- at an MOI of 10. Total RNA was extracted and 1μg in 1μl was spotted onto a nylon membrane, UV-crosslinked and processed like a Western blot. DsRNA was detected by staining with the dsRNA-specific antibody J2. B) Quantification of dsRNA in mock-infected HeLa and HFF cells from 5 independent experiments, * pC) Quantification of dsRNA signal in HSV- and mock-infected HFF cells from 6 independent experiments, * pD) Quantification of dsRNA signal in HSV- and mock-infected HeLa cells from 3 independent experiments, * p<0.05.</p