Vhs and US11 make independent and complementary contributions to PKR suppression during HSV infection.

Abstract

HFF and HeLa cells were infected for 12 hours with HSV-1 (KOS37) WT, Vhs-, US11-, Vhs-US11-, IEUS11 and IEUS11 Vhs- at an MOI of 10. A) Whole-cell lysates were analyzed by Western blot with the indicated antibodies. Note that the Vhs antibody used cross-reacts with a co-migrating cellular protein present in HFF cells. B) Quantification of the P-PKR signal in HFF and HeLa cells. Data are from 4 independent experiments. The P-PKR signal in cells infected with Vhs-US11- was set at 100%.</p