AphA and HapR abundances vary inversely during biofilm formation.

Abstract

(A) Representative image series showing the formation of an individual biofilm harboring the constitutive reporter PTAC-mRuby3 and AphA-mNG. (B) Quantitation of the AphA-mNG fluorescence (black line) relative to the control mRuby3 fluorescence (magenta line) over the course of biofilm development. n = 24 biofilms from three biological replicates. (C and D) As in A and B, respectively, for HapR-mNG. Inset in (D) represents the AphA-mNG/HapR-mNG ratio over time. Shading in B and D represents SD. Numerical data are available in S1 Data. a.u., arbitrary unit; mNG, mNeonGreen.</p