MOESM1 of Apelin-13/APJ system attenuates early brain injury via suppression of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation and oxidative stress in a AMPK-dependent manner after subarachnoid hemorrhage in rats

Abstract

Additional file 1: Table S1 Study design and animal usage. Table S2. Rats physical data after surgeries. Figure S1. The ventral side was divided into six parts. SAH severity score: a grade from 0-3 is dependent on the amount of blood clot in each segment as follows: grade 0:no blood clot; 1: minimal blood clot; 2: moderate blood clot with recognizable arteries; 3: blood clot obliterating all arteries. The SAH grade was the total scores of the six parts, with minimal score of 0 and maximal score of 18. Figure S2. Effects of apelin-13 on brain edema: the quantification of brain water content of cerebellum and brain stem at 24 h after SAH. Figure S3. Depletion Efficiency of APJ siRNA with Naïve Rats. (A) Representative Western blot images. (B) Quantitative analyses of APJ. n=6 for each group. The bars represent the mean ± SD. *p<0.05 versus naïve