Far-red light intensity controls biofilm formation.

Abstract

(A) Colony biofilm phenotypes are shown for WT PA14 and the designated mutants on Congo red agar medium after 72 h of growth under the indicated far-red light intensities. Scale bar is 2 mm for all images. (B) Relative expression of rpoD (squares) and pelA (circles) measured by qRT-PCR in WT PA14 (black) and in the bphPSTOP mutant (green) grown as colony biofilms as in (A). Data were normalized to 16S RNA levels, and the WT levels at 0 mW/m2 far-red light were set to 1.0. Error bars represent SEM for 3 biological replicates. Data for panel B can be found in supplemental file S1 Data. AU, arbitrary unit; qRT-PCR, quantitative Reverse Transcriptase-Polymerase Chain Reaction; SEM, standard error of the mean; WT, wild type.</p