Re-analysis of HPLC peaks previously assigned as phenazines from original data collected on an older HPLC.

Abstract

UV–vis chromatograms are shown on the left, and the full UV–vis spectra of the peaks indicated with an arrow are shown on the right. Where indicated on the y-axis, the data have been normalized to bring the data into the same scale for clarity. (A) Analysis of the PCA peak, comparing a pure PCA standard (chromatogram at 364 nm, orange) to a sputum sample (chromatogram at 398 nm, black). (B) Analysis of the pyocyanin peak, comparing pyocyanin in P. aeruginosa-free sputum (blue) to a P. aeruginosa-positive sputum sample (black) (both chromatograms at 387 nm).</p