Relative transcript levels of putative genes after paraquat treatment.

Abstract

A. Gene transcript levels in susceptible (S) and resistant (R) goosegrass at the indicated times after paraquat treatment. Transcripts were detected by real-time PCR using total RNA extracted from 10-d-old seedlings sprayed with 10/HM paraquat. Error bars represent the SD (n = 3). B. Amplification products of 4 putative genes from goosegrass by RT-PCR. For the M:DL 2000 marker, “-” indicates the negative control. R0, R30, R60, R90, R120, and R180 indicate samples of R goosegrass seedlings taken 0, 30, 60, 90, 120 and 180 min after paraquat treatment, while S0, S30, S60, S90, S120 and S180 indicate samples of S goosegrass seedlings taken 0, 30, 60, 90, 120 and 180 min after paraquat treatment, respectively.</p