Triple color FISH identifies mucosal bacteria in the adherent mucus compartment of dogs with IBD following prednisone and dietary therapy.

Abstract

Panels A-C = ileal tissues and panels D-F = colonic tissues. Panel A = probe Cy3-Faecali698; Panel B = probe Cy3-Ebac; Panel C = probe Cy3- Strc493; Panel D = probe Cy3-Bif164; Panel E = Cy3-Ebac; Panel F = probe FITC-Eub338. All other bacteria that hybridize exclusively with the universal probe (Eub338-FITC) appear green. DAPI-stained intestinal mucosa with goblet cells appears blue. All images at 600x magnification.</p