The schematic diagram of the experimental procedure.

Abstract

EVs in human plasma samples were first isolated using anti-CD63 antibody-coated magnetic beads. Captured EVs were then eluted for whole particle assays, such as transmission electron microscopy and nanoparticle tracking analysis. In addition, captured EVs were lysed for studying their molecular contents, including proteins and RNAs. EV-miRNAs were extracted using oligonucleotide-conjugated magnetic beads and subjected to subsequent analysis.</p