Arf6 is related to Ang II-induced human podocyte injury.

Abstract

A. Human podocytes were treated with angiotensin II (Ang II) as indicated. Apoptosis was evaluated with caspase 3 activity. Data are presented as mean ± SD. n = 5. *p .01 vs. 0 h or as indicated comparison. B and C. Human podocytes were treated with 1 μM of Ang II for 12, 24 and 48 h, respectively. The mRNA (B) and protein (C) levels of Arf6 was assessed and compared. Data are presented as mean ± SD. n = 3. *p S1 Fig. D. Human podocytes were treated with 1 μM of Ang II for 24 h, and indirect immunofluorescence was performed for Arf6 staining (green color). The non-treated cells were used as the controls (CTL). The 2nd antibody only was used for the blank control. Magnification x 40.</p