Ang II increases ROS production through Nox4 in human podocyte.

Abstract

A. Human podocytes were treated with 1 μM of angiotensin II (Ang II) for 12, 24, and 48 h, and the protein levels of Nox4 were assessed and compared. Data are presented as mean ± SD. n = 3. *p .01 vs. 0 h. B and C. Podocytes stably expressing siRNA-Nox4 (siNox4) or siRNA-control (siCTL) were treated with 1 μM of Ang II for 24 h. The non-treated podocytes were used as the controls (CTL). The protein levels of Nox4 (B) and the ROS (C) were assessed and compared. Data are presented as mean ± SD. n = 3 (B) and 6 (C). *p .01 vs. CTL, #p .05 vs. Ang II or Ang II + siCTL. D and E. Podocytes were transduced with 15 μl of lentiviral shRNA-Arf6 (shArf6) and the control shRNA (shCTL), respectively. The non-treated cells were used as the controls (CTL). The protein levels of Nox4 and Arf6 (D) and the ROS level (E) were assessed. Data are presented as mean ± SD. n = 3 (D) and 4 (E). *p .01 vs. CTL, #p .05 vs. Ang II or Ang II + shCTL. Full length blots of A, B and D are provided in S3 Fig.</p