Abstract

Additional file 2. Flow chart of multi-level PCR analysis for detection of squirrel polyomaviruses. Generic nested VP1 PCR (second-round product displayed as magenta-coloured bar) with degenerate primers was conducted. For full genome amplification, this was followed by specific nested long-distance PCR (LD-PCR; second-round product of approximately 5 kbp shown as red bar) and overlapping standard nested PCR (second-round product of approximately 800 bp shown as green bar) with specific primers. Grey bars represent coding sequences, black bar the non-coding control region

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