MOESM1 of Alpha-mangostin induces endoplasmic reticulum stress and autophagy which count against fatty acid synthase inhibition mediated apoptosis in human breast cancer cells

Abstract

Additional file 1: Figure S1. The effects of α-mangostin on ER stress, autophagy, cell viabilities in MCF-7 cells. (A) MCF-7 cells were treated with 0, 1, 2, and 4 μM α-mangostin for 24 h, and then the relative expression levels of CHOP, BIP, LC3II/LC31 and P62 were analyzed by western blot and were quantified densitometrically with the software ImageJ and calculated according to the reference bands of GAPDH. Data represented the mean ± SD of three independent experiments. **p < 0.01. (B) MCF-7 cells were treated with 4 μm α-mangostin, 5 mM 4PBA, 5 mM 3MA or a combination of them. Cell viabilities were then determined by the CCK-8 assay. Data represented the mean ± SD of three independent experiments. **p < 0.01. (C) MCF-7 cells were treated with/without 4 μm α-mangostin followed 24 h incubation with/without 10 μM PA. Cell viabilities were then determined by the CCK-8 assay. Data represented the mean ± SD of three independent experiments. **p < 0.01