MOESM3 of Alpha-mangostin induces endoplasmic reticulum stress and autophagy which count against fatty acid synthase inhibition mediated apoptosis in human breast cancer cells

Abstract

Additional file 3: Figure S3. α-Mangostin inhibited intracellular FAS activity and reduced the amount of free fatty acids. (A) MDA-MB-231 cells were treated with 0, 1, 2, and 4 μM α-mangostin for 24 h, then intracellular FAS activity was determined spectrophotometrically by measuring the decrease of absorbance at 340 nm due to oxidation of NADPH. (B) MDA-MB-231 cells were treated with 0, 1, 2, and μM α-mangostin for 24 h. Then cells were harvested using trypsin–EDTA, washed twice with PBS. Intracellular fatty acid was determined with a Free Fatty Acid Quantification Kit (Bivision) according to the manufacturer’s instructions. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01