MOESM2 of Alpha-mangostin induces endoplasmic reticulum stress and autophagy which count against fatty acid synthase inhibition mediated apoptosis in human breast cancer cells

Abstract

Additional file 2: Figure S2. The time-dependent effects of α-mangostin on ER stress and autophagy in MDA-MB-231 cells. Cells were treated with 4 μm α-mangostin for 0, 6, 12, 18, and 24 h, and then the relative expression levels of CHOP, BIP, LC3II/LC31 and P62 were analyzed by western blot and were quantified densitometrically with the software ImageJ and calculated according to the reference bands of GAPDH. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01