UGT1A1 catalyzed the demethoxycurcumin-<i>O</i>-glucuronidation, and expression of UGT1A1 and efflux transporters in HeLa1A1 cells.

Abstract

(A) Kinetic profiles for demethoxycurcumin-O-glucuronides (G1 and G2) (0.5–40 μM) by UGT1A1; (B) Kinetic profiles for demethoxycurcumin-O-glucuronides (G1 and G2) (0.5–40 μM) by HeLa1A1 cell lysate; In each panel, the insert figure showed the corresponding Eadie-Hofstee plot. (C) Protein expression of UGT1A1, BCRP and four MRP family transporters in HeLa and HeLa1A1 cells. All experiments were performed in triplicate (n = 3). Data were expressed as mean ± SD (n = 3).</p