MRP3 silencing led to reduce excretion rates and increase intracellular demethoxycurcumin-<i>O</i>-glucuronides in HeLa1A1 cells.

Abstract

(A) MRP3 transporter silencing inhibited the accumlated demethoxycurcumin-O-glucuronides (G1) in extracellular medium; (B) MRP3 transporter silencing decreased the excreted demethoxycurcumin-O-glucuronides (G2) in extracellular solution; (C) MRP3 silencing significantly reduced the excretion rates of G1 and G2; (D) MRP3 silencing resulted in evaluation of intracellular G1 and G2 levels; (E) MRP3 silencing decreased total cellular glucuronidation (G1 ang G2) of demethoxycurcumin; (F) Effects of gene silencing on the protein level of MRP3. The concentration of demethoxycurcumin was 4 μM. All experiments were performed in triplicate (n = 3). Data were presented as mean ± SD. *, # p , ## p , ### p < 0.001 compared with that of control group of G1 or G2, respectively.</p