Additional file 1: of Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression

Abstract

Figure S1 Isolated germline nuclei from a second germline transgenic strain. (A) Young adult pie-1p::GFP::H2B transgenic worm showing germline expression. GFP::H2B is expressed in the nuclei of germ cells at all developmental stages, including oocytes and embryos. (B) Isolated nuclei from pie-1p::GFP::H2B young adult worms were immunostained with GFP (green) and stained with DAPI (blue). Nuclei stained with both GFP and DAPI (89.1%, n = 2111) were considered germline nuclei. Two independent experiments were performed. (C) Isolated nuclei from wild type VC2010 N2 young adult worms were immunostained with GFP (green) and stained with DAPI (Blue). Scale bars, 20 μm in (A), 5 μm in (B) and (C). (PDF 6536 kb