MOESM1 of Arabidopsis and rice showed a distinct pattern in ZIPs genes expression profile in response to Cd stress

Abstract

Additional file 1: Table S1. The qPCR primers used in this study. Table S2. Primers used in plasmid construction. Figure S1. Genome locations of 27 ZIP genes in Arabidopsis (A) and rice (B). Information were acquired in the PLAZA database and plotted using Photoshop CS6. Figure S2. Evolutionary relationships of ZIP family genes and their structures. The Neighbor-Joining tree was produced using MEGA7 with 1,000 bootstrap replicates, and the gene structures was predicted using Gene Structure Display Server. Dark blue boxes indicate exons; black lines indicate introns; light blue boxes indicate untranslated regions. Figure S3. Effect of Cd stress on root length (A and B) and dry weight (C and D) of Arabidopsis and rice. (for root length, n=20; for dry weight, n=3. Student t test, * indicates P<0.05). Figure S4. Effect of ZIPs on Cd accumulation. Cells expressing ZIPs were incubated using liquid YPD medium plus 300 μg/mL G418 and 50 μM Cd for 12 h, after which the Cd concentration of each strain was measured by an atomic absorption spectrometer method. Cells harboring empty pCEV-G1-Km (Vector) was used as a negative control. (n=3, student t test, * P < 0.05)