Additional file 6: of Food perception without ingestion leads to metabolic changes and irreversible developmental arrest in C. elegans

Abstract

Figure S4. Perception and physiological effects of potential food cues. (A) The proportion of larvae that recovered to at least the L4 stage after 3 days of recovery is plotted for three biological replicates. (B-C) GFP::DAF-16 localization is plotted for three to six biological replicates. (D) L1 starvation survival is plotted over time. A logistic regression of mean survival from three biological replicates is shown. (E) Worm length following 48 h of recovery is plotted relative to L1 starvation survival. (F) Worm length following 48 h of recovery is plotted as a density plot, showing altered population composition. (A-E) ***p < 0.001, **p < 0.01, *p < 0.05; unpaired t test. Error bars are SEM, except for in E where they are standard deviation. (A) Quadruple mutant transgenic background. (B-F) Wild-type background. (PDF 93 kb