Additional file 2: of An improved suppression subtractive hybridization technique to develop species-specific repetitive sequences from Erianthus arundinaceus (Saccharum complex)

Abstract

Figure S2. Electrophoresis of primary PCR products after SSH. M: 100 bp marker; Lanes 1–4: products of subtracted first PCR by 62 °C, 64 °C, 66 °C, 68 °C. 10 μM primers and 0.5 U LA Taq polymerase (a), 10 μM primers and 1 U LA Taq polymerase (b), 20 μM primers and 0.5 U LA Taq polymerase (c). (TIF 12107 kb