raw movie data

Abstract

ZIP-compressed hierarchical folder tree containing raw movie data as 16 bit TIFF image stack files. Each movie was acquired by fluorescence video microscopy on compartmentalized motor neurons cultured in Xona microfluidic chambers at standardized distal and proximal axonal readout positions at 3 frames per second with Lysotracker and Mitotracker. For more details refer to Methods, section ‘Live imaging of MN in MFCs’. The file path of the hierarchical folder tree defines the metadata of each movie (i.e. the experimental conditions of each movie) and serves as the base for the automated movie annotation with KNIME software, as detailed in Methods, section ‘Handling of meta data throughout the work flow’ and ‘Data mining in CSV result files and assembly of final EXCEL result tables with KNIME'